DocumentsDate added
Case report and review of literature
Jigar M Dhuvad1,*,Rajesh A Kshirsagar2,Mukesh M Dhuvad3
Affiliation:
1Assistant Professor, Government Dental College and Hospital, Ahmedabad, India.
2Professor and Head, Bharati Vidyapeeth Dental College and Hospital, Pune, India
3Private practiconer, Ahmedabad, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Oral and Maxillofacial Surgery, Bharati Vidyapeeth Dental College and Hospital, Pune, India
Address reprint requests to
* Dr Jigar M Dhuvad (MDS)
D-34; Ishan-1; Behind Seema Hall, Satellite, Ahmedabad, India or at drjigardhuvad1981@gmail.com
Article citation: Dhuvad JM, Kshirsagar RA, Dhuvad MM. Mucormycosis: An opportunistic fungal infection in uncontrolled diabetic patients: Experience of two cases and a review of literature. J Pharm Biomed Sci. 2015; 05(07):583-588. Available at www.jpbms.info
ABSTRACT: Mucormycosis, also known as zygomycosis, is an opportunistic fulminant fungal infection, which mainly affects the immunocompromised patients. The infection begins in the nose and paranasal sinuses due to inhalation of fungal spores. It has six predominant clinical forms: rhinocerebral, central nervous system, pulmonary, cutaneous, gastrointestinal, disseminated, and uncommon (i.e., heart, bone, kidney, mediastinum, bladder, and oral). Although oral involvement of this condition has been reported relatively frequently in the literature, we report two cases of mucormycosis in an uncontrolled diabetic patient. Because of its rapid progression and high mortality, early diagnosis of this potentially fatal fungal infection along with aggressive treatment offers the only chance to increase the survival rates. The purpose of this article is to report our clinical experience with two cases of mucormycosis of the maxilla in relation with uncontrolled diabetes mellitus and a review of the literature in relation to various types of mucormycosis.
KEYWORDS: Central nervous system; fungal infections; maxilla; mucormycosis.
REFERENCES
1.George Petrikkos, Anna Skiada, Olivier Lortholary, Emmanuel Roilides, Thomas J. Walsh, and Dimitrios P. Kontoyiannis. Epidemiology and Clinical Manifestations of Mucormycosis. Clinical Infectious Diseases 2012; 54:S23–34.
2.Prabhu RM, Patel R. Mucormycosis and entomophthoramycosis: a review of the clinical manifestations, diagnosis and treatment. Clin Microbiol Infect 2004; 10:31–47.
3.Ribes JA, Vanover-Sams CL, Baker DJ. Zygomycetes in human disease. Clin Microbiol Rev 2000; 13:236–301.
4.Paulltauf A: Mycosis mucorina. Virchows Arch A 1885; 102:543.
5.A Ghafur, PS Shareek, Nambi P Senthur, PR Vidyalakshm, V Ramasubramanian Ashok parameswaran, MA Thirunarayan, et al. Mucormycosis in Patients without Cancer: A Case Series from a Tertiary Care Hospital in South India. J of the asso of physic of India 2013; 61: 305-08.
6.Arunaloke Chakrabarti, Manpreet Dhaliwal. Epidemiology of Mucormycosis in India. Current Fungal Infection Reports 2013; 7: 287-92.
7.Hajdu S, Obradovic A, Presterl E: Invasive mycoses following trauma. Injury 2009; 40:548.
8.Kavitha Prasad, R. M. Lalitha, E. K. Reddy, K. Ranganath, D. R. Srinivas, and Jasmeet Singh. Role of Early Diagnosis and Multimodal Treatment in Rhinocerebral Mucormycosis: Experience of 4 Cases. J Oral Maxillofac Surg 2012; 70:354-362.
9.O’Neill BM, Alessi AS, George EB, Piro J. Disseminated rhinocerebral mucormycosis: a case report and review of literature. J Oral Maxillofac Surg 2006; 64:326-33.
10.Anita Pandey, Vishal Bansal, Ashish K. Asthana, Vikas Trivedi, Molly Madan, Anupam Das. Case Report: Maxillary osteomyelitis by mucormycosis: report of four cases. Intern J of Infec Dis 2011; 15: e66–69.
11.Schütz P, Behbehani JH, Khan ZU, et al: Fatal rhino-orbitocerebral zygomycosis caused by Apophysomyces elegans in a healthy patient. J Oral Maxillofac Surg 2006; 64:1795.
12.Mantadakis E, Samonis G: Clinical presentation of zygomycosis. Clin Microbiol Infect 2009; 15:15.
13.Eggimann P, Garbino J, Pittet D: Epidemiology of Candida species infections in critically ill non-immunosuppressed patients. Lancet Infect Dis 2003; 3:685.
14.Stefano Viterbo, Massimo Fasolis, Paolo Garzino-Demo, Alessandro Griffa, Paolo Boffano, Caterina Iaquinta, et al. Management and outcomes of three cases of rhinocerebral mucormycosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112:e69-74.
15.Brad Spellberg, John Edwards Jr and Ashraf Ibrahim. Novel Perspectives on Mucormycosis: Pathophysiology, Presentation,and Management. Clin microbio Rev 2005; 18: 556–69.
16.Pillsbury, H. C., and N. D. Fischer. Rhinocerebral mucormycosis. Arch. Otolaryngol. 1977; 103:600–04.
17.Marr, K. A., R. A. Carter, F. Crippa, A. Wald, and L. Corey. Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients. Clin. Infect. Dis 2002; 34:909–17.
18.McNulty, J. S. Rhinocerebral mucormycosis: predisposing factors. Laryngoscope 1982; 92:1140–43.
19.Hosseini SM, Borghei P. Rhinocerebral mucormycosis: pathways of spread. Eur Arch Otorhinolaryngol 2005; 262:932-38.
20.Adam, R. D., G. Hunter, J. DiTomasso, and G. Comerci, Jr. Mucormycosis: emerging prominence of cutaneous infections. Clin. Infect. Dis 1994; 19:67–76.
21.Kerr, O. A., C. Bong, C. Wallis, and M. J. Tidman. Primary cutaneous mucormycosis masquerading as pyoderma gangrenosum. Br. J. Dermatol 2004; 150:1212–13.
22.Mead, J. H., G. P. Lupton, C. L. Dillavou, and R. B. Odom. Cutaneous Rhizopus infection. Occurrence as a postoperative complication associated with an elasticized adhesive dressing. JAMA 1979; 242:272–74.
23.Petrikkos G., A. Skiada, H. Sambatakou, A. Toskas, G. Vaiopoulos, M. Giannopoulou, and et al. Mucormycosis: ten-year experience at a tertiary-care center in Greece. Eur. J. Clin. Microbiol. Infect. Dis 2003; 22:753–56.
24.Woods, K. F., and B. J. Hanna. Brain stem mucormycosis in a narcotic addict with eventual recovery. Am. J. Med 1986; 80:126–28.
25.Maliwan, N., C. V. Reyes, and J. W. Rippon. Osteomyelitis secondary to cutaneous mucormycosis. Report of a case and a review of the literature. Am. J. Dermatopathol 1984; 6:479–81.
26.Leong, A. S. Y. Granulomatous mediastinitis due to Rhizopus species. Am. J. Clin. Pathol 1978; 70:103–07.
27.Pierce P. F., M. B. Wood, G. D. Roberts, R. H. Fitzgerald, Jr., C. Robertson, and R. S. Edson. Saksenaea vasiformis osteomyelitis. J. Clin. Microbiol 1987; 25:933–35.
28.Connor, B. A., R. J. Anderson, and J. W. Smith. Mucor mediastinitis. Chest 1979; 75:524–26.
29.Andrews, D. R., A. Allan, and R. I. Larbalestier. Tracheal mucormycosis. Ann. Thorac. Surg 1997; 63:230–32.
30.Welk B., A. A. House, E. Ralph, E. Tweedy, and P. P. Luke. Successful treatment of primary bilateral renal mucormycosis with bilateral nephrectomy. Urology 2004; 64:590.
31.Helenglass, G., J. A. Elliott, and N. P. Lucie. An unusual presentation of opportunistic mucormycosis. Br. Med. J. (Clin. Res. ed.) 1981; 282:108–09.
32.Rothstein, R. D., and G. L. Simon. Subacute pulmonary mucormycosis. J. Med. Vet. Mycol. 1986; 24:391–94.
33.VK Jha, PJ Borpujari, G Shenoy, S Bhargav. Empyema with Pleuropulmonary Mucormycosis: case report. J of the association of physic of India 2013; 61: 77-79.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Copyright © 2015 Dhuvad JM, Kshirsagar RA, Dhuvad MM. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review article
*Ranjan Bhattacharyya. MD,DNB (Psychiatry)
Affiliation:
Assistant Professor, Department of Psychiatry, Murshidabad Medical College & Hospital, Berhampore, West Bengal, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Psychiatry, Murshidabad Medical College & Hospital, Berhampore, West Bengal, India
Address reprint requests to
*Dr Ranjan Bhattacharyya.
Assistant Professor, Department of Psychiatry, Murshidabad Medical College & Hospital, Berhampore, West Bengal, India or at +91-9433053389/94755565090
Article citation: Bhattacharyya R. Living with the Corpse: Introspection Amidst the Media Hype. J Pharm Biomed Sci. 2015; 05(07):551-563. Available at www.jpbms.info
ABSTRACT: Introduction: The police officials on 11 th June, 2015 recovered a charred body of a 77-year-old man, a fully-clothed skeleton of a woman in bedrooms, two bags full of bones of dogs with only living person, a man in his mid-40s in the house in Kolkata. Methodology: The event has been described as the reports collected from media sources, police and forensic evidences. Discussion: The event has been described on the basis of psychopathology with expert reviews. Conclusion: The media role has been highlighted along with an attempt had been made to generate more awareness about mental illness removing the stigma associated with it.
KEYWORDS: Notes and diaries; Forensic aspects; Media reporting; Necrophilia; Shared delusional disorder.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
REFERENCES
1.http://www.hindustantimes.com/kolkata/skeletons-in-closet-kolkata-man-lived-with-skeletons-of-daughter-and-2-pet-dogs/article1-1357431.aspx
2.http://www.gettyimages.in/detail/news-photo/dr-pradip-saha-directer-of-the-institute-of-psychiatry-dr-news-photo/477326202
3.https://www.facebook.com/sayanb360
4.http://www.abplive.in/india/2015/06/12/article616384.ece/Kolkatas-Alfred-Hitchcock-house-My-sister-too-is-at-home-dead
5.http://www.indiatimes.com/news/india/sex-stories-and-violence-7-of-the-most-disturbing-things-found-at-the-kolkota-house-of-horrors-233588.html
6.http://www.firstpost.com/india/horror-house-robinson-street-hidden-notes-strange-sex-stories-emerge-kolkata-investigation-2297400.html
7.http://timesofindia.indiatimes.com/city/kolkata/Bizarre-sex-tales-found-in-Kolkatas-House-of-Horror/articleshow/47650977.cms
8.Goodwin R, Cramer D. Inappropriate Relationships: The Unconventional, the Disapproved, and the Forbidden. Psychology Press2002;174–76.
9.Weismantel M. Moche sex pots: Reproduction and temporality in ancient South America". American Anthropologist 2004;106: 495–96.
10.Adler A, Magruder WW. Folie à deux in identical twins treated with electro-shock therapy. Journal of Nervous and Mental Disease. 1946;103:181–186.
11.Apostolo A, Vignati C, Brusoni D, Cattadori G, Contini M, Veglia F, Agostoni P. Erectile dysfunction in heart failure: Correlation with severity, exercise performance, comorbidities, and heart failure treatment. Journal of Sexual Medicine. 2009;6:2795–2805.
12.Arnone D, Patel A, Tan G. The nosological significance of folie à deux: A review of the literature. Annals of General Psychiatry. 2006;5:11
13.Joshi KG, Frierson RL, Gunter TD. Shared psychotic disorder and criminal responsibility: A review and case report of folie a trois. Journal of the American Academy of Psychiatry and the Law. 2006;34:511–17.
14.Newman WJ, Harbit MA. Folie à deux and the courts. Journal of the American Academy of Psychiatry and the Law. 2010;38:369–75.
15.http://pro.psychcentral.com/dsm-5-changes-schizophrenia-psychotic-disorders/004336.html
16.http://www.docplexus.in/articles/39d9da21-8ed9-4e9c-b85f-ced6caadb54e last accessed on 22/6/15.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Copyright © 2015 Bhattacharyya R. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Research article
Moumita Mondal1,*, Swamya Soman1,*, Renukaradhya K. Math1,§
Affiliation:
1,*Master of Science in Biotechnology, §Ph.D Department of Biotechnology, Garden City College, 16 KM, Old Madras Road, Bangalore-560049, India
The name of the department(s) and institution(s) to which the work should be attributed:
Department of Biotechnology, Garden City College, 16 KM, Old Madras Road, Bangalore-560049, India
Address reprint requests to
* Renukaradhya K. Math.
Department of Biotechnology, Garden City College, 16 KM, Old Madras Road, Bangalore-560049, India
*Moumita and Swamya contributed equally
Article citation: Mondal M, Soman S, Math RK. Evaluation Of antibacterial activity Of methanol and acetone extracts (different parts) of moringa oleifera and hibiscus rosasinensis. J Pharm Biomed Sci. 2015; 05(07):532-538. Available at www.jpbms.info
ABSTRACT: Methanol and acetone extract of two plant genus; Moringa oleifera and Hibiscus rosa-sinensis was carried out to identify the particular part of plant having antibacterial activities and also analyze phytochemicals constituents. Crude extracts were tested for antibacterial activity with concentrations of 200 and 300μg/ml. Acetone extracts of Moringa oleifera seed (MOS) and Moringa oleifera fruit pulp (MOFP) showed significant antibacterial activity against test organisms; for S.aureus, P.aeroginosa at 20 μg/ml and for E.coli, and S.typhi at 300μg/ml. While, methanol extracts of MOS and MOFP also shown activity against test organism except against S.typhi at 300μg/ml. Meanwhile, among Hibiscus rosa-sinensis leaf (HRL), Hibiscus rosa-sinensis flower petal (HRFP) and Hibiscus rosa-sinensis flower clayx (HRFC), only HRFC of acetone and methanol extracts shown significant activity at 300 μg/ml against S.aureus, E.coli and P.aeroginosa but no activity was shown against S.typhi. Surprisingly, methanol and acetone extracts of leaf extracts from both the plants did not show any activity on test organism. Subsequently, phytochemical analysis of acetone extracts supposed that compounds like, methionine, moringine, nitriles, isothiocynate, thiocarbonates and saponins might be involved in inhibiting the bacterial growth. Thus, seeds and pulp of M. oleifera & calyx of H. rosa-sinensis can be used as potential sources to extract antimicrobial agents possessing medicinal values.
KEYWORDS: Moringa oleifera; Hibiscus rosa-sinensis; organic solvents; antimicrobial activity; phytochemicals.
REFERENCES
1.Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J. Ethnopharmacol. 2004; 93: 1-7.
2.Machado TB, Pinto AV, Pinto MCFR, Leal CR, Silva MG, Amaral ACF, Kuster RM, Netto–dosSantos KR. Invitro activity of Brazilian medicinal plants, naturally occurring naphthoquinones and their analogues, against methicillin-resistant staphylococcus aureus. Int. J. Antimicrobial Agents, 2003; 21: 279-284.
3.Pandey A, Kaushik A, Tiwari SK. Evaluation of antimicrobial activity and phytochemical analysis of Citrus limon. J. Pharma. BioMedical Sci. 2011, 13 (17).
4.Rathi B, Pathi PA, Baheti AM. Evaluation of aqueous extract of pulp and seeds of Moringa oleifera for wound healing in albino rats. J. Natural Remedies, 2004; 4: 145-149.
5.Singh GP, Sharma SK. Antimicrobial evauation Moringa Oleifera Int.Res.J.Pharmacy 2012;3:(4).
6.Gerson S. The Foundation for Holistic Medical Research, Brewster, New York; 2002.
7.Bushnell OA, Fukuda M, Makinodian T. The antibacterial properties of some plants found in Hawaii. Pacific Sci. 1950; 4: 167-183.
8.Duke, JA, Ayensu, ES. Medicinal Plants of China. 2 Vols. 705 S., 1300 Strichzeichnungen. Reference Publ., Inc. Algonac. Michigan, 1985. ISBN 0-917266-20-4.
9.Chopra RN, Nayer A,Chopra IC. Glossary of Indian Medicinal plants, (including the supplement). Council of Scientific and Industrial Research, (CSIR), New Delhi, 1986.
10.Nadkarni KM. Indian Materia Medica, With Ayurvedic, Unani-Tibbi, Siddha, Allopathic, Homeopathic, Naturopathic & Home Remedies, Appendices & Indexes. Popular Prakashan, Bombay. 1976, 40–43
11.Shivananda NB, Sivachandra RS, Orette FA, Chalapathi RAV. Effects of Hibiscus rosa-sinensis L (Malvaceae) on wound healing activity: A preclinical study in a Sprague Dawley rat. Int. J. Low Extrem. Wounds, 2007; 6(2): 76-81.
12.Gauthaman KK, Saleem MTS, Thanislas PT, Prachul VV, Krishnamoorthy KK, Devaraj NS, and Somasunsaram JS. Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat. BMC Comp. Alt. Med. 2006; 6: 32.
13.Yamasaki H, Uefuji H, and Sakihama Y. Stress proteins and mycocardial protection. Arch. Biochem. Biophys. 1996; 332: 183-186.
14.Harbone JB. Phytochemical methods, A guide to modern techniques of Plant analysis, Chapman and Hall, New York, 1984; 85.
15.Khandelwal KR. Practical Pharmacognosy, Nirali Prakshan, Pune, 2004, 149-156.
16.Tripathi P, Patel JR. “Hepatoprotective activity of Ficus lacor buchan”. Int. J. Pharmacol. Biol. Sci. 2007; 1(1): 33-35.
17.Patel P, Patel N, Patel D, Desai S, Meshram D. Phytochemical analysis and antifungal activity of moringa oleifera. Int. J. Pharm. Pharm. Sci. 2014, 6(5): 144-147.
18.Selvam P, Raj K, Vimisha V, Harikrishnan R, Sarija KS, Umalekshmi R. Antimicrobial Activity of Fruit Extracts of Morinda citrifolia. J. Appl. Chem. Res. 2009; 10: 61-63.
19.Usha R, Sashidharan S, Palaniswamy M. Antimicrobial Activity of a Rarely Known Species, Morinda citrifolia L. Ethnobotanical Leaflets, 2010; 14: 306-311.
20.Karaman I, Sahin F, Gulluce M, Ogutcu H, Sengul M, Adiguzel A. Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J. Ethnopharma. 2003; 2837: 1-5.
21.Wei LS, Musa N, Sengm CT, Wee W, Mohd Shazili NA. Antimicrobial properties of tropical plants against 12 pathogenic bacteria isolated from aquatic organisms. African J. Biotechnol. 2008; 7:2275-2278.
22.Sharma V, Paliwal R. Isolation and characterization of saponins from Moringa Oleifera (Moringaeceae) pods. Int. J. Pharm. and Pharmaceut. Sci. 2013; 5: 179-183.
23.Jayaraman SK, Manoharan MS, Illanchezian S. Antibacterial, antifungal and tumor cell suppression potential of Morinda citrifolia fruit extracts. Int. J. Integ. Biol. 2008; 3: 44-49.
24.Parekh J, Chanda S.V. In vitro antimicrobial activity and phytochemical analysis of some indian medicinal plants. Turk. J. Biol. 2007; 31: 53-58.
25.Shukla YN, Mishra M. A hydroxyacid and sterol from hibiscus rosa sinensis. Indian Drugs. 2001; 38: 543.
26.Siddiqui AA, Wani SM, Rajesh R, and Alagarswamy V. Phytochemical and pharmacological investigation of flowers of hibiscus rosasinensis linn. Indian J. Pharm. Sci. 2006; 68: 127-130.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: Garden City Education Trust, Bangalore-560049, India
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Copyright © 2015 Mondal M, Soman S,Math RK. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
Shetgar Anupama1, Patil Brijesh2, Anandi Vinut3 ,Gill Kanwarpal Singh4
Affiliation:
1Assistant Professor, Department of Ophthalmology, 2Associate Professor, Department of Ophthalmology, 3Resident, Department of Ophthalmology,4Resident, Department of Ophthalmology S.Nijalingappa Medical College, Bagalkot, Karnataka, India
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Ophthalmology S.Nijalingappa Medical College, Bagalkot, Karnataka, India
Abstract:
Aim: To determine the epidemiological characteristics and risk factors predisposing to infective keratitis in Bagalkot, Karnataka, India.
Methods: All patients with suspected infective keratitis attending ophthalmology outpatient department at Hanagal Shri Kumareshwar hospital from First of October 2014 were accessed. Sociodemographic data and risk factors were recorded. Corneal scrapings and samples were sent for microbiological assessment.
Results: In 3 month period 50 cases with infective keratitis were documented and accessed. 64% males being the majority with middle aged working population as prime section.
65% had predisposing trauma as history with 43% attended hospital within 1st week of event.76% sought medical help. Among them 52% had fungal pathogen, 43% had bacterial pathogen, 5% had mixed aetiological agents.
Conclusion: Infective keratitis being the commonest cause for corneal opacity, most oftenly occurs after an episode of trauma with agricultural products or tools.
KEYWORDS: Infective keratitis; Risk factors; Health facility; Bagalkot.
REFERENCES
1.Srinivasan M et al. Epidemiology and aetiological diagnosis of corneal ulceration in Madurai,S outh India.Br J Ophthalmology 1997;8:965-71.
2.Leck AK,Thomas PA, Hagan M,Kaliamurthy J,Ackuaku E,John M, et al. Aetiology of suppurative corneal ulcers in Ghana and South India,and epidemiology of fungal keratitis, Br J Ophthalmology2002;86:1211-15.
3.Bharati MJ,Ramakrishna R,Vasu S,Meenakshi R,Palaniappan R,Epidemological Characteristics and Laboratory diagnosis of fungal keratitis:a three year study. Indian J Ophthalmol 2003;51:315-21.
4.Sharma S,Athmanathan T. Diagnostic procedures in infectious keratitis. In: Nema HV, Nema N,editors. Diagnostic procedures in Ophthalmology. Jaypee Brothers medical publishers. New delhi;2002.pp232-53.
5.Dutta LC,Dutta D,Mohanty P, Sharma J. Study of fungal keratitis. Indian J Ophthalmol 1981;29:407-09.
6.Gonzales CA,Srinivasan M,Whitcher JP, Smolin G,Incidence of corneal ulceration in Madurai district,southindia.Ophthalmic epidemiol1996;3:159-66.
7.Thylefors B. Epidemiological patterns of ocular trauma.Aust NZ J Ophthalmol 1992;20;95-8.
8.Courtright P,Lewallen S,Kanjaloti S,Dighton D.Traditional eye medicine use among patients with corneal disease in rural.Malawi. Br J Ophthalmol 1994;78:810-2.
9.Basak S,Basak S,Mohanta A, Bhowmick A. Epidemiological and Microbiological diagnosis of suppurative keratitis in gangetic west Bengal,Eastern india.Indian J Ophthalmol 2005;53:17-22.
Statement of Originality of work: The manuscript has been read and approved by all the authors, the requirements for authorship have been met, and that each author believes that the manuscript represents honest and original work.
Source of funding: None
Competing interest / Conflict of interest: The author(s) have no competing interests for financial support, publication of this research, patents and royalties through this collaborative research. All authors were equally involved in discussed research work. There is no financial conflict with the subject matter discussed in the manuscript.
Disclaimer: Any views expressed in this paper are those of the authors and do not reflect the official policy or position of the Department of Defense.
Majority of the information gathered are from media sources which don’t reflect the author’s own opinion.
Copyright © 2015 Shetgar A, Patil B, Anandi B,Gill KS. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Original article
O. C. Akanji1,* and M. A. Sonibare2
Affiliation:
1Department of Plant Science and Biotechnology, Adekunle Ajasin University, Akungba Akoko, Nigeria.
2Department of Pharmacognosy, University of Ibadan, Ibadan, Nigeria.
The name of the department(s) and institution(s) to which the work should be attributed:
1.Department of Plant Science and Biotechnology, Adekunle Ajasin University, Akungba Akoko, Nigeria
2.Department of Pharmacognosy, University of Ibadan, Ibadan, Nigeria
Address reprint requests to
* O. C. Akanji.
Department of Plant Science and Biotechnology, Adekunle Ajasin University, Akungba Akoko, Nigeria
Article citation: Akanji OC, Sonibare MA. Effect of Erythrophleum Suaveolens (Guill. & Perr.) Brenan on normal and infected wounds. J Pharm Biomed Sci. 2015; 05(07):519-523. Available at www.jpbms.info
ABSTRACT: Aim of study: To investigate dichloromethane, ethyl acetate and aqueous fractions of methanolic extract of Erythrophleum suaveolens stem-bark for normal wound healing and infected wound healing using excision wound model in Wistar rats as a follow up to an initial study of crude extract.
Methods: Local infection was introduced into rat abdominal wounds with Staphylococcus aureus and Pseudomonas aeruginosa using a 108 cells/ml inoculum. Rate of wound healing was assessed by wound contraction and period of epithelization.
Results: The aqueous fraction treated wounds were found to epithelize faster and the rate of wound contraction was significantly increased as compared to control wounds. The test drug achieved 100% wound contraction on day 14 in normal wounds, on day 12 in Staphylococcus aureus wounds and on day 14 in Pseudomonas aeruginosa wounds.
Conclusions: The results indicated that the aqueous fraction of Erythrophleum suaveolens stem-bark promotes wound healing significantly and proves to be a potential ant-infective agent.
KEYWORDS: Wound healing; epithelization; Erythrophleum Suaveolens; infection; excision wound.
References:
1.Akanji OC and Sonibare MA. Evaluation of Wound Healing Activity of Erythrophleum suaveolens (Guill. & Perr.) Brenan and Moringa oleifera Lam. On Infected Albino Rats. EJMP.2015: 7(2): 67-76.
2.Ambia S, Narayana R, Gowri D, Sukumar D, Madhavan S. Evaluation of wound healing activity of flavonoids from Ipomea carnea Jacq. 2007;26(3):45-51.
3.Bowler PG, Deurden BI, Armstrong DG. Wound microbiology and associated approach in wound management. Clin Microbiol Rev. 2001;14:244-9.
4.Bucknall TE. The effect of local infection upon wound healing: An experimental study. Brit J Surg. 1980; 67:851-855.
5.Calabrese V, Scapagnini C, Catalano C, Dinotta F, Geraci D, Morganti P Biochemical studies of a natural antioxidant isolated from rosemary and its application in cosmetic dermatology. Int. J. Tissue React. 2000;22: 5-13.
6.Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-582
7.Dissemond J, Goos M, Wagner SN. The role of oxidative stress in the pathogenesis and therapy of chronic wounds. Hautaryt. 2002; 53:718-23.
8.Hollinworth H. The management of infected wounds. Professional Nurse Study 1997;12:8-11.
9.Houghton PJ, Mensah AY, Hensel A, Deters AM. In vitro tests and ethnopharmacological investigations: Wound healing as an example. J. Ethnopharmacol. 2005;100:100-7.
10.Kanchanapoom T, Kamel MS, Picheansoonthon C, Luecha P, Kasai R, Yamasaki K.Hydrolyzable tannins and phenylpropanoid from Rafflesia kerrii Meijer (Rafflesiaceae). J Nat Med. 2007;61:478–479.
11.Mahmood AA, Abdalbasit AM, Siddig IA, Salmah I, Fouad AB. Potential activity of ethanolic extract of Boesenbergia rotunda (L.) rhizomes extract in accelerating wound healing in rats. J. Med. Plants Res. 2010;4(15):1570-1576.
12.Nayak BS, Sandiford S, Maxwell A. Evaluation of the wound healing activity of Ethanolic extract of Morinda citrifolia leaf. Evidence – Based Complement. Alternat. Medicine. 2009;6(3):351-356.
13.Neuwinger HD. Deutsche Apotheker Zeitung.1998;16:44.
14.Olugbuyiro JAO. Antimicrobial and wound healing properties of Flabellaria paniculata and Rothmannia longiflora. MSc Thesis, University of Ibadan, Ibadan.
15.Padamaja, PN, Bairy, KL and Kulkarni DR (1994). Pro-healing effect of betel nut and its Polyphenols. Fitoterapia. Vol. 65(2): 299.
16.Palanicharmy S, Bhaskar EA, Bakthvath salam, R and Nagarajan, S (1991) Wound healing activity of Cassia alata. Fitoterapia Vol. 62 (2): 154.
17.Saurez J, Herreta MD, Marhuenda E. Hesperidine and neohesperidine dihydrochalcone on different experimental models of induced gastric ulcer. Phytother Res. 1996;10:616–618.
18.Sidhu GS, Mani H, Gaddipatti JP,Singh AK,Seth P,Banaudha KK. Cucumi enhances wound healing in streptozotocin induced diabetic rats and genetically diabetic mice. Wound repair and regeneration.1991;7:362-74.
19.Tally FP and Gorbachi SL. Anaerobic Bacteriology for Clinical Laboratories. Tufts Univ. School of Medicine, Boston. 1980;51
20.Buckill HM (1995). The useful plants of West Africa, Vol. 3 and 42nd edition. Royal Botanic Gardens, Kew pp3, 606-609.
21.Udegbunam SO,Nnaji TO.Udegbunam R. I. Okafor J. C. and Agbo I. Evaluation of herbalointment formulation of Milicia excels (Wetw) C. C. berg for wound healing,Afr. J. Biotechnol., 2013:12(21), pp. 3351-3359
22.Reddy J.S,Rao PR,Reddy MS,(2002). Wound healing effects of Heliotropium indicum, Plumbago zeylanicum and Acalypha indica in rats. Ethnopharmacology 79, 249-251.
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